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Engineering Creativity for Neuroscience

 

 

In-line Solution Heaters

 

We have developed these sophisticated In-line Solution Heaters to regulate the temperature of the incoming solution to brain slice chambers and living tissue chambers. In-line Solution Heaters can be used in any microelectrode laboratory (e.g. patch clamp). Our In-line Solution Heaters are designed to be used in shielded environment, because they do not generate any hum or other noise. In-line Solution Heaters are the best warming devices of brain slice chambers and living tissue chambers. Chamber heaters regulate the temperature of the whole liquid content of the chamber, except the most important area, above the objective. The objectives are not heated in most cases. That is, why immersion objectives behave as rude heat drains. As a result, the observed cell culture will be much colder than the surrounding solution. Even the most important area is warmed improperly by chamber heaters. In-line Solution Heaters solve this issue.

 

    Click on the picture to get it in full size

 

There are two different versions of  the In-line Solution Heaters in our choice. The single-channel version contains one heated tube and the dual-channel version contains two, independent heated tubes. The two tubes of the dual-channel model can be used independently, either with different flow rates. Our In-line Solution Heaters are able to warm up on very high flow rates compared to the competitors.

 

The outer diameter of the tube connectors are 1.2 mm. The thickness of the wall of the tubes are 0.114 mm. The internal stainless steel metal tubing is made of a very special material. This kind of steel alloy is fully inert for any biological solutions.

 

The internal tubing of the In-line Solution Heaters form Archimedean spirals (also called arithmetic spiral). This geometry of the tube provides the linear reduction of the speed of the solution from the input to the output tube connectors. It is an essential feature, because it minimizes the number of the oxygen bubbles at the output liquid flow. According to this construction the working position of the In-line Solution Heater is the perfect horizontal position. The side with the blue factory logo label and the heat sink should be positioned as top side. The input tube connector is that one, which is located next to the mechanical fixing connector. These positioning rules are important for proper operation.

 

In the In-line Solution Heaters there are internal temperature sensors. It is recommended to use them instead of the temperature sensors immersed in the solution baths. See the explanation about the advantages of the internal temperature sensors on the Temperature Management in Physiology and in Biology product description page.

 

There are different connection points to ground and/or shield of the equipments in every lab. The low voltage signal ground of the Physiological - Biological Temperature Controller is connected to its back-side aluminium plate and the heat sink at the back side of the Temperature Controller. During the construction of the In-line Solution Heaters we have focused on the highest noise suppression and the versatile shielding and grounding possibilities. For this purpose the internal assembly of the In-line Solution Heater units are NOT connected to the signal ground of the Temperature Controller. The In-line Solution Heater units are totally floating! This feature gives you a full freedom to connect them anywhere electrically. The best connection points are the stainless steel tubes, because they can be reached easily and they are connected to every internal metal parts of the In-line Solution Heaters. The tubes can be clamped with crocodile clips. The best point, where to connect the heater to ground is the low voltage signal (and local shield) ground point, as close to the chamber as possible. The appropriate ground point, where to connect, should be chosen according to several different aspects. But there are two reasons, why it is strongly recommended to connect the In-line Solution Heater to ground:

1) Suppression of hum noise in the shielded environment

2) Elimination of the parasitic electrochemical (galvanic) potentials between the different metal parts

 

    Click on the picture to get it in full size

 

To use an In-line Solution Heater you should buy a Physiological - Biological Temperature Controller assembled with 12V FINE DC power end-stage. Either for the Single-channel In-line Solution Heater or for the Dual-channel In-line Solution Heater only one piece of single-channel Physiological - Biological Temperature Controller is enough to operate.

 

The plastic enclosure is not hermetically sealed. Please ensure not to pour liquid on the enclosure. Please do not immerse the equipment into water, saline or any other solution. The sealing of this equipment has limitations. The electronic circuit will go wrong if corrosive solution diffuses into the plastic case.

 

 

Single-channel In-line Solution Heater

 

Flow rate: 12 ml / min (specified for 37 Centigrade at the output tube)

 

Internal solution buffer volume: 5.2 ml

 

Hysteresis: less than +/- 0.4 Centigrade

 

Outer dimensions of the plastic box: 90 x 50 x  25 mm (excluding the heat sink)

 

    Click on the picture to get it in full size

 

 

Dual-channel In-line Solution Heater

 

Flow rate: 2 x 7 ml / min (specified for 37 Centigrade at the output tube)

 

Internal solution buffer volume: 2 x 5.2 ml

 

Hysteresis: less than +/- 0.5 Centigrade

 

Outer dimensions of the plastic box: 90 x 50 x  32 mm (excluding the heat sink)

 

    Click on the picture to get it in full size

 

 

Lockable Magnetic Fixing Stand

 

Lockable Magnetic Fixing Stand is an independently orderable option for the In-line Solution Heaters. Lockable Magnetic Fixing Stand has got flexible, back bone styled holder arm on its magnetic basement.  The magnetic basement has also a lever to lock it on the desired place on any ferromagnetic steel surface. This device provides a very stable, but versatile fixing method. The flexible holder arm can be locked in any position by a small lever. This way you can install the In-line Solution Heater in optimal position in any experimental environment easily. The connection force of the basement of the fixing stand to the ferromagnetic surface beneath it is 800 N.

 

    Click on the picture to get it in full size

 

 

Replacement

 

Since the cell cultures and other biological objects are very sensitive, the In-line Solution Heater should not be used if it is injured or any internal dirt or infection appeared in it. For such cases, which happen in every lab during normal usage, we have a special offer, only for our previous customers. If you send your used In-line Solution Heater back to Supertech Instruments, we will change it for a brand new piece. A used In-line Solution Heater could not be renewed, because all the internal parts should have been changed. Usually the plastic enclosure and the electric cable is also worn-out. So we can not reuse any part of the old piece at all. We need it back, because we disassemble it to discover the effects of the usage on the internal elements. This way we collect valuable hidden information to improve the quality of the product. We provide this replacement possibility on a discount price.

 

 

Warranty Limitation

 

Supertech Instruments gives you 5 years of full warranty for all electronics products, including the In-line Solution Heaters. But the warranty does not cover the faults made by the user. The warranty is valid for the In-line Solution Heaters except for:

- broken stainless steel tube

- bent tube

- plugging in the tube

- dirt in the tube

- infection in the tube

- corrosion in the electronic heater or sensor circuit

- broken wire

 

 

Tips

 

The internal area of the tubes of the In-line Solution Heaters can be cleaned efficiently by passing 20% citric acid solution through the tubes. The time of the cleaning process and the necessary quantity of the citric acid solution depends on the level of dirtiness. During the washing action it is suggested to heat up the In-line Solution Heater for 40 Centigrade.

 

Based on the experience obtained in Dr. Norbert Hajos' laboratory (Hajos et al., European Journal of Neuroscience, Vol. 29, pp. 319-327, 2009, email: hajos@koki.hu), the temperature and the oxygenation of brain slices in a Dual-Superfusion Slice Chamber Insert could be controlled in two ways. Either the experiments should be done at room temperature, then only the flow rate of aCSF (Artificial CerebroSpinal Fluid) has to be set equivalently below and above the slices (e.g. using peristaltic pumps with at least three channels - two inlets and one outlet). Or, in the case, when experiments are desired to perform at higher temperature, then not only the flow rate but the temperature, as well, should be maintained as equivalent as possible below and above the slices. To achieve this, a Dual-channel In-line Solution Heater is recommended to use, where the temperature of two inlets is controlled in a single heater head to ensure the same temperature.

 

 

Prices

 

You can find the general warranty and shipping conditions in the beginning of the Price List page.

 

Single-channel In-line Solution Heater (one heated tube) without lockable magnetic fixing stand (compatible with the 12V Physiological - Biological Temperature Controller): 340 EUR

 

Dual-channel In-line Solution Heater (two independent tubes in one equipment) without lockable magnetic fixing stand (compatible with the 12V Physiological - Biological Temperature Controller): 480 EUR

 

Replacement Single-channel In-line Solution Heater (for the case of injury, internal dirt or infection) without stand, only for previous customers, if we get the worn-out piece back: 300 EUR

 

Replacement Dual-channel In-line Solution Heater (for the case of injury, internal dirt or infection) without stand, only for previous customers, if we get the worn-out piece back: 430 EUR

 

Lockable Magnetic Fixing Stand, 800 N force: 90 EUR

 

Physiological - Biological Temperature Controller, up to 50 Centigrade (0.1 C resolution): you can look up its price on the Temperature Management in Physiology and in Biology page.

 

 

 

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